BIOTECHNOLOGY FACILITY
Tissue Culture Lab (Pilot Basis)
Established: 2015
KVK Pal, Jalgaon-I
6 min read
Tissue Culture Lab | Disease-Free, Uniform, High-Yielding Plantlets
Infrastructure
Washing Area
Glassware cleaning
Media Preparation
Nutrient media mixing
Sterilization Room
Autoclave for sterilization
Inoculation Room
Laminar air flow for sterile transfer
Culture Room
25±2°C, light 16/8 hours
Hardening Greenhouse
Acclimatization of plantlets
Species Micropropagated
Hardwickia binnata (Anjan)
Tectona grandis (Teak - Clonal)
Banana (Grand Naine, Robusta)
Gerbera
Orchids
Carnation
Production Process (Stages of Micropropagation)
Stage 0
Selection of elite mother plant
Stage 1: Initiation
Explant (shoot tip/meristem) surface sterilized and placed on MS medium
Stage 2: Multiplication
Multiple shoots induced using cytokinins (BAP). Multiplication rate: 3-5x per subculture (every 4 weeks)
Stage 3: Rooting
Shoots transferred to medium with auxin (IBA/NAA)
Stage 4: Hardening
Plantlets transferred to pro-trays with cocopeat in shade net for 4-6 weeks
Production Capacity & Cost
Production Capacity
50,000-1,00,000 plantlets per year
Cost to Farmer
₹8-12 per plantlet (market price ₹15-25)
Advantage over Conventional: Disease-free, uniform growth, higher yield (25-30% more), available year-round.
Advantage over Conventional
- Disease-free planting material
- Uniform growth and maturity
- Higher yield (25-30% more) compared to conventional methods
- Available year-round irrespective of season
- Virus-free and genetically uniform plants
